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Biotechnic & histochemistry : official publication of the Biological Stain Commission | Vol.81, Issue.2-3 | | Pages 105-6

Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation.

P C, Peters C, Thoni E J, Harry

Abstract

A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when using 2-photon excitation, Alexa 488 was rapidly and substantially photobleached. The unexpected instability of Alexa 488 under certain conditions may have deleterious consequences if not anticipated and accommodated in experimental protocols.

Original Text (This is the original text for your reference.)

Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation.

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Keywords

visible immunofluorescence microscopy alexa 488

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P C, Peters C, Thoni E J, Harry,.Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation.. 81 (2-3),105-6.

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