T-cell receptor (TCR) delta gene repertoire, as assessed by V delta-J delta rearrangements, has been analyzed in nine multiple sclerosis (MS) cases and in 30 healthy individuals by seminested PCR technique. Among the V delta-J delta junctional diversities studied, the most striking result has been observed in V delta 5-J delta 1 rearrangement. The detection of repeated V delta 5-J delta 1 nucleotide sequences in all analyzed clones from seven out of nine patients studied proved the monoclonal nature of gamma delta T-cells with V delta 5-J delta 1 rearrangement. The clonal nature of this rearrangement proved by PAGE and sequencing analysis may suggest an antigen-driven expansion of gamma delta T cells and argues for a significant role of gamma delta T-cells with V delta 5-J delta 1 rearrangement in MS pathogenesis. However, it cannot be excluded that clonal expansion of these lymphocytes may represent secondary change to central nervous system damage.

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sequencing gamma delta t cells receptor tcr delta gene repertoire nature clonal expansion seminested pcr antigendriven v deltaj delta junctional diversities page

J S, Nowak G, Micha?owska-Wender D, Januszkiewicz M, Wender,.Limited junctional diversity of V delta 5-J delta 1 rearrangement in multiple sclerosis patients.. 30 (1-2),95-100.

J S, Nowak G, Micha?owska-Wender D, Januszkiewicz M, Wender,"Limited junctional diversity of V delta 5-J delta 1 rearrangement in multiple sclerosis patients." 30

J S, Nowak G, Micha?owska-Wender D, Januszkiewicz M, Wender,"Limited junctional diversity of V delta 5-J delta 1 rearrangement in multiple sclerosis patients."