The kinetics of ultrasonically-induced cell lysis are examined in terms of classical radiation biology target theory. A theoretical expression relating the concentration of intact cells remaining after a given period of sonication in a rotating culture tube to the number of non-trapped bubbles, l, which a cell must encounter in order to be lysed is obtained. The expression is compared to experimental results in order to determine the actual value of l. It is found that l equals one (1). The concentration of non-trapped bubbles which is responsible for the observed cell lysis is calculated to be 250-500 cm-3. Finally, it is proposed that non-trapped bubbles tunnel into cells while undergoing stable cavitation and that cell lysis is produced by one or more transient events inside the cell.

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radiation biology nontrapped bubbles l stable cavitation ultrasonicallyinduced cell lysis culture

C C, Church M W, Miller,.The kinetics and mechanics of ultrasonically-induced cell lysis produced by non-trapped bubbles in a rotating culture tube.. 9 (4),385-93.

C C, Church M W, Miller,"The kinetics and mechanics of ultrasonically-induced cell lysis produced by non-trapped bubbles in a rotating culture tube." 9

C C, Church M W, Miller,"The kinetics and mechanics of ultrasonically-induced cell lysis produced by non-trapped bubbles in a rotating culture tube."