Electrophoretic displacement of charged entity phase modulates the spectrum acquired in electrophoretic NMR experiments, and this modulation can be presented via 2D FT as 2D mobility spectroscopy (MOSY) spectra. We compare in various mixed solutions the chemical selectivity provided by 2D MOSY spectra with that provided by 2D diffusion-ordered spectroscopy (DOSY) spectra and demonstrate, under the conditions explored, a superior performance of the former method. 2D MOSY compares also favourably with closely related LC-NMR methods. The shape of 2D MOSY spectra in complex mixtures is strongly modulated by the pH of the sample, a feature that has potential for areas such as in drug discovery and metabolomics. Copyright © 2016 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd. Two-dimensional mobility spectroscopy (MOSY) spectra are obtained by displaying the signal modulation in electrophoretic NMR experiments via 2D FT. The chemical selectivity provided by 2D MOSY spectra compares favourably with that provided by 2D DOSY spectra in various mixed solutions. As an extra advantage, 2D MOSY spectra are strongly modulated by the pH of the sample.

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2d mosy spectra signal modulation chemical selectivity metabolomics 2d diffusionordered spectroscopy electrophoretic nmr drug discovery shape sample displacement of charged entity phase resonance

Pavel V. Yushmanov, István Furó,Yuan Fang,.Assessing 2D electrophoretic mobility spectroscopy (2D MOSY) for analytical applications. 55 (6),588-584.

Pavel V. Yushmanov, István Furó,Yuan Fang,"Assessing 2D electrophoretic mobility spectroscopy (2D MOSY) for analytical applications" 55

Pavel V. Yushmanov, István Furó,Yuan Fang,"Assessing 2D electrophoretic mobility spectroscopy (2D MOSY) for analytical applications"