1. 1. Female sperm antagglutin has been isolated from the follicle fluid of the cow by adsorption on a column of quartz powder and fractionated elution with water saturated with carbon dioxide. 2. 2. The substance, which is not a proteid like the male equivalent, is free from nitrogen, sulphur, and phosphorus. It contains non-reducing sugars and a carbocyclic residue, showing similarities to tocopherols and present also in male antagglutin. 3. 3. The female antagglutin is very soluble in water, soluble in methanol and ethanol, and slightly soluble in ethyl ether, acetone, and carbon tetrachloride. 4. 4. The female antagglutin gives positive reaction with the procedure elaborated by Lindahl, Kihlström, and Ross (1957) for the quantitative determination of male antagglutin. Applied to human cervical mucus, this reaction is positive during the ovulation phase, and negative in the later part of the cycle. 5. 5. The biological activity referred to concentration shows an asymptotic course with lower values of the asymptote for purified antagglutin than for cruder preparations, e.g. cervical mucus. The antagglutin is protected against oxidation in the follicle fluid and the cervical mucus.

+More

follicle nonreducing sugars carbocyclic residue quantitative determination of male antagglutin human cervical mucus nitrogen

P.E. Lindahl and A. Nilsson,.The isolation of sperm antagglutin from the follicle fluid, and some of its properties. 25 (1),22-32.

P.E. Lindahl and A. Nilsson,"The isolation of sperm antagglutin from the follicle fluid, and some of its properties" 25

P.E. Lindahl and A. Nilsson,"The isolation of sperm antagglutin from the follicle fluid, and some of its properties"