Meiotic recombination plays a critical role in sexual reproduction by creating crossovers between homologous chromosomes. These crossovers, along with sister chromatid cohesion, connect homologs to enable proper segregation at Meiosis I. Recombination is initiated by programmed double strand breaks (DSBs) at particular regions of the genome. The meiotic recombination checkpoint uses meiosis-specific modifications to the DSB-induced DNA damage response to provide time to convert these breaks into interhomolog crossovers by delaying entry into Meiosis I until the DSBs have been repaired. The meiosis-specific kinase, Mek1, is a key regulator of meiotic recombination pathway choice, as well as being required for the meiotic recombination checkpoint. The major target of this checkpoint is the meiosis-specific transcription factor, Ndt80, which is essential to express genes necessary for completion of recombination and meiotic progression. The molecular mechanism by which cells monitor meiotic DSB repair to allow entry into Meiosis I with unbroken chromosomes was unknown. Using genetic and biochemical approaches, this work demonstrates that in the presence of DSBs, activated Mek1 binds to Ndt80 and phosphorylates the transcription factor, thus inhibiting DNA binding and preventing Ndt80's function as a transcriptional activator. Repair of DSBs by recombination reduces Mek1 activity, resulting in removal of the inhibitory Mek1 phosphates. Phosphorylation of Ndt80 by the meiosis-specific kinase, Ime2, then results in fully activated Ndt80. Ndt80 upregulates transcription of its own gene, as well as target genes, resulting in prophase exit and progression through meiosis.

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interhomolog dna binding dsbs genome meiosisspecific transcription factor ndt80 dsbinduced dna damage response meiotic recombination checkpoint transcriptional modifications double strand meiosisspecific kinase mek1 sexual reproduction

Xiangyu Chen,Robert Gaglione,Trevor Leong,Lauren Bednor,Teresa de Los Santos,Ed Luk,Michael Airola,Nancy M Hollingsworth,.Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80.. 14 (11),.

Xiangyu Chen,Robert Gaglione,Trevor Leong,Lauren Bednor,Teresa de Los Santos,Ed Luk,Michael Airola,Nancy M Hollingsworth,"Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80." 14

Xiangyu Chen,Robert Gaglione,Trevor Leong,Lauren Bednor,Teresa de Los Santos,Ed Luk,Michael Airola,Nancy M Hollingsworth,"Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80."