Background/Aims: Recent evidence has shown that some long non-coding RNAs (lncRNAs) play important roles in various biological processes. However, the regulatory mechanism of lncRNA in gastric cancer (GC) remains unclear. Methods: We reannotated the GC gene expression profile into a lncRNA-mRNA biphasic profile and integrated the microRNA target data to construct a global GC triple network. A further clustering and random walk with restart analyses was performed on the triple network from the level of topology analyses. Quantitative real-time PCR was used to determine expression of lncRNA RP11-363E7.4. Kaplan-Meier analyses was performed to evaluate the prognostic value of lncRNA RP11-363E7.4. Results: We constructed a gastric cancer lncRNA-miRNA-mRNA network (GCLMN) including six lncRNAs, 332 mRNAs, and 3,707 edges. For the shared lncRNA RP11-363E7.4, the interacting gene and microRNA functional enrichment studies implied that lncRNA RP11-363E7.4 might function as a new regulator in GC. The expression of lncRNA RP11-363E7.4 was downregulated compared with that of paracarcinoma tissues in five GC samples. High expression of lncRNA RP11-363E7.4 was found to be correlated to better overall survival (OS) for GC patients. Conclusions: This study focused on GC lncRNA-miRNA-mRNA regulatory networks, and found that lncRNA RP11-363E7.4 was a new GC risk lncRNA, which might provide novel insight into a better understanding of the pathogenesis of GC.

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random walk with restart analyses gc gene expression profile lncrnamrna biphasic profile lncrnas 332 mrnas 3707 interacting gene microrna functional enrichment global gc triple network gastric cancer lncrnamirnamrna network realtime pcr microrna target data lncrna rp11363e74 gc lncrnamirnamrna regulatory networks paracarcinoma long noncoding rnas

Pengcheng Wang,Jing Li,Wei Zhao,Chunyang Shang,Xian Jiang,Yuli Wang,Baoguo Zhou,Fusheng Bao,Haiquan Qiao,.A Novel LncRNA-miRNA-mRNA Triple Network Identifies LncRNA RP11-363E7.4 as An Important Regulator of miRNA and Gene Expression in Gastric Cancer. 47 (3),.

Pengcheng Wang,Jing Li,Wei Zhao,Chunyang Shang,Xian Jiang,Yuli Wang,Baoguo Zhou,Fusheng Bao,Haiquan Qiao,"A Novel LncRNA-miRNA-mRNA Triple Network Identifies LncRNA RP11-363E7.4 as An Important Regulator of miRNA and Gene Expression in Gastric Cancer" 47

Pengcheng Wang,Jing Li,Wei Zhao,Chunyang Shang,Xian Jiang,Yuli Wang,Baoguo Zhou,Fusheng Bao,Haiquan Qiao,"A Novel LncRNA-miRNA-mRNA Triple Network Identifies LncRNA RP11-363E7.4 as An Important Regulator of miRNA and Gene Expression in Gastric Cancer"