Conventional reconstruction methods used in cryo-EM single particle analysis do not take the depth of field effect into account. Here the authors present a block-based reconstruction method to deal with the depth of field effect and show that this approach can improve the resolution of cryo-EM virus structures.

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cryoem single particle analysis blockbased reconstruction method resolution cryoem virus structures depth of field effect

Dongjie Zhu,Xiangxi Wang,Qianglin Fang,James L Van Etten,Michael G Rossmann,Zihe Rao,Xinzheng Zhang,.Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. 9 (1),.

Dongjie Zhu,Xiangxi Wang,Qianglin Fang,James L Van Etten,Michael G Rossmann,Zihe Rao,Xinzheng Zhang,"Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions" 9

Dongjie Zhu,Xiangxi Wang,Qianglin Fang,James L Van Etten,Michael G Rossmann,Zihe Rao,Xinzheng Zhang,"Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions"