Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on the autophagy-regulating effect of TBP-2 under oxidative stress in human lens epithelial cells (LECs). Human lens epithelial cells were used for cell culture and treatment. Lentiviral-based transfection system was used for overexpression of TBP-2. Cytotoxicity assay, western blot analysis, GFP/mCherry-fused LC3 plasmid, immunofluorescence, and transmission electronic microscopy were performed. The results showed that autophagic response of LECs with increased LC3-II, p62, and GFP/mCherry-LC3 puncta (P

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gfpmcherrylc3 puncta p cellular antioxidant autophagic autophagyregulating lc3ii p62 lecs negative regulator of thioredoxin trx tbp2 cytotoxicity transmission electronic microscopy blot analysis gfpmcherryfused lc3 plasmid immunofluorescence

Jiaojie Zhou,Yidong Zhang,Guangdi Chen,Kairan Lai,Yibo Yu,.Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation. 2016 (),.

Jiaojie Zhou,Yidong Zhang,Guangdi Chen,Kairan Lai,Yibo Yu,"Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation" 2016

Jiaojie Zhou,Yidong Zhang,Guangdi Chen,Kairan Lai,Yibo Yu,"Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation"