In vertebrates, skeletal myogenesis involves the sequential activation of myogenic factors to lead ultimately to the differentiation into slow and fast muscle fibers. How transcriptional co-regulators such as arginine methyltransferases PRMT4/CARM1 and PRMT5 control myogenesis in vivo remains poorly understood. Loss-of-function experiments using morpholinos against PRMT4/CARM1 and PRMT5 combined with in situ hybridization, quantitative polymerase chain reaction, as well as immunohistochemistry indicate a positive, but differential, role of these enzymes during myogenesis in vivo. While PRMT5 regulates myod, myf5 and myogenin expression and thereby slow and fast fiber formation, PRMT4/CARM1 regulates myogenin expression, fast fiber formation and does not affect slow fiber formation. However, our results show that PRMT4/CARM1 is required for proper slow myosin heavy chain localization. Altogether, our results reveal a combinatorial role of PRMT4/CARM1 and PRMT5 for proper myogenesis in zebrafish.

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myod myf5 and myogenin expression hybridization quantitative polymerase chain reaction transcriptional coregulators skeletal myogenesis prmt5 myogenic factors zebrafish myogenin expression fast fiber formation arginine methyltransferases prmt4carm1 slow myosin heavy chain localization

,.The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish.. 6 (10),.

,"The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish." 6

,"The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish."