In this article, we elaborate the application of thermal decomposition based synthesis of Fe₃O₄ superparamagnetic nanoparticle (SPMNP) in subcellular fractionation context. Here, we performed surface functionalization of SPMNP with phospholipids and dimercaptosuccinic acid. Surprisingly, we observed surface functionalization dependent SPMNP localization in subcellular compartments such as plasma membrane, endosomes and lysosomes. By using SPMNP based subcellular localization with pulse–chase methodology, we could use SPMNP for high pure-high yield organelle (plasma membrane, endosomes and lysosome) fractionation. Further, SPMNP that are distinctly localized in subcellular compartments can be used as technology for subcellular fractionation that can complement existing tools for cell biology research. As a future perspective, isolated magnetic organelles can be extended to protein/protein complex purification for biochemical and structural biology studies.

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thermal decomposition based synthesis surface functionalization dependent spmnp localization cell biology lysosome fractionation further spmnp membrane endosomes complex purification subcellular fractionation spmnp based subcellular localization with pulsechase fesub3subosub4sub superparamagnetic nanoparticle spmnp biochemical and structural biology magnetic organelles

Deepak B. Thimiri Govinda Raj,Niamat Ali Khan,.Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome. 5 (1),.

Deepak B. Thimiri Govinda Raj,Niamat Ali Khan,"Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome" 5

Deepak B. Thimiri Govinda Raj,Niamat Ali Khan,"Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome"