The intracellular calcium ([Ca2+]i) response induced by external forces can be diverse and complex. Using primary osteoblasts from Wistar rats, we found multiple patterns of [Ca2+]i responses induced by fluidic shear stress (Fss), including homogeneous non-oscillation and heterogeneous oscillations. These multiple-patterned [Ca2+]i responses could be influenced by Fss intensity, cell density, and cell differentiation. Our real-time measurements with free calcium, ATP, ATP without calcium, suramin, apyrase, and thapsigargin confirmed homogeneous [Ca2+]i patterns and/or heterogeneous [Ca2+]i oscillations with respect to the combined degree of external Ca2+ influx, and intracellular Ca2+ release. Our theoretical model supported diverse Fss-induced calcium activities as well. We concluded that a singular factor of Ca2+ influx or release dominated to produce smooth homogeneous patterns, but combined factors produced oscillatory heterogeneous patterns. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res

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intracellular calcium ca2i response fss intensity cell density heterogeneous ca2i oscillations suramin apyrase cell differentiation smooth homogeneous patterns degree fssinduced calcium activities fluidic shear stress external ca2 influx multiplepatterned ca2i responses

Cunbo Li, Haiying Sun,Guixian Meng, Imshik Lee,.Multiple calcium patterns of rat osteoblasts under fluidic shear stress. 35 (12),.

Cunbo Li, et al."Multiple calcium patterns of rat osteoblasts under fluidic shear stress" 35

Cunbo Li, Haiying Sun,Guixian Meng, Imshik Lee,"Multiple calcium patterns of rat osteoblasts under fluidic shear stress"