A probe-type oxygen sensor was developed utilizing a radioluminescent (RL)-based light source and a ruthenium-based sensing chemistry for monitoring the dissolved oxygen (DO) concentration in a modified version of the NASA-designed high aspect ratio vessel (HARV), a batch rotating wall vessel. This sensor provided the means to monitor the DO concentration in the HARV without influencing the flow pattern, thereby retaining the low shear HARV environment conducive to the formation of 3-dimensional cell aggregates. This sensor lost significant signal as a result of exposure to the first three autoclave cycles, but only minimal change in signal was observed following exposure to subsequent autoclave cycles. A new calibration model requiring only one fitted parameter was developed that accurately fit data over the entire range from 0% to 100% oxygen saturation. The ability for DO concentration control within the vessel was demonstrated by using this sensor to monitor the DO concentration inside the HARV.

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radioluminescent rlbased light source rutheniumbased sensing chemistry probetype oxygen sensor shear harv dissolved oxygen do concentration 0 3dimensional cell high aspect ratio vessel batch rotating wall calibration model

Mark A, Saarinen Julie S, Reece Mark A, Arnold David W, Murhammer,.Monitoring and controlling the dissolved oxygen (DO) concentration within the high aspect ratio vessel (HARV).. 19 (4),1335-41.

Mark A, Saarinen Julie S, Reece Mark A, Arnold David W, Murhammer,"Monitoring and controlling the dissolved oxygen (DO) concentration within the high aspect ratio vessel (HARV)." 19

Mark A, Saarinen Julie S, Reece Mark A, Arnold David W, Murhammer,"Monitoring and controlling the dissolved oxygen (DO) concentration within the high aspect ratio vessel (HARV)."