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Indian journal of medical microbiology | Vol.21, Issue.1 | | Pages 17-20

Search for Shiga toxin producing Escherichia coli (STEC) including 0157: H7 strains in and around Kolkata.

U K, Chattopadhyay S, Gupta S, Dutta

Abstract

An attempt was made to isolate and characterize Shiga toxin producing E. coli (STEC) from animals handlers, animal products and admitted diarrhoeic children in and around Kolkata.A total of 415 samples were processed for detection of STEC by PCR and colony hybridization techniques.0 (4.81%) samples were found to be positive for STEC. Diarrhoeic cattle accounted for maximum (22.1 %) isolation.The study showed PCR to be more sensitive than hybridization technique for detection of STEC.

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Search for Shiga toxin producing Escherichia coli (STEC) including 0157: H7 strains in and around Kolkata.

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Keywords

shiga toxin hybridization technique stec pcr e coli

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U K, Chattopadhyay S, Gupta S, Dutta,.Search for Shiga toxin producing Escherichia coli (STEC) including 0157: H7 strains in and around Kolkata.. 21 (1),17-20.

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