A method of construction of amplified cDNA libraries was developed on the basis of selective inhibition of cDNA amplification and modified for the studied models for analysis of expression of the genes containing LeR-1 and VeR-1 sequences. Time-related changes in expression of these genes were studied during regeneration of the adult lens and during embryogenesis of newts. The pattern of expression of the LeR-1 and VeR-1 genes proved to be not tissue-specific. A fragment adjoining 5'-area of the LeR-1 gene was obtained using a new approach: rapid amplification of cDNA ends by polymerase chain reaction (5'-RACE-PCR). Analysis of the LeR-1 clone primary structure using Gene Bank did not show essential homology with the known nucleotide sequences. Sequence LeR-1 is characterized by evolutionary conservation of genomic DNA in Pleurodeles waltlii, Xenopus laevis, and Rana temporaria.

+More

clone ler1 and ver1 sequences amplified cdna libraries polymerase chain reaction sequences sequence inhibition of cdna amplification analysis of expression pleurodeles waltlii xenopus laevis and rana temporaria ler1 gene bank

Iu V, Markitantova K A, Luk'ianov O V, Kazanskaia V I, Mitashov S A, Luk'ianov,.[An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts].. 28 (4),262-70.

Iu V, Markitantova K A, Luk'ianov O V, Kazanskaia V I, Mitashov S A, Luk'ianov,"[An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts]." 28

Iu V, Markitantova K A, Luk'ianov O V, Kazanskaia V I, Mitashov S A, Luk'ianov,"[An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts]."