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Voprosy medit?sinsko? khimii | Vol.34, Issue.5 | | Pages 124-9

Voprosy medit?sinsko? khimii

[Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test].

G M, Rott G M, Lapshina I V, Rozhinskaia O A, Letova T V, Blinova  
Abstract

Optimal conditions were developed for estimation of myoglobin using immunoenzyme assay (ELISA) of competitive and "sandwich" types. Informative efficiency of the ELISA procedures was compared with commercially available hemagglutination test "Erythrocyte diagnosticum for myoglobin detection; dry immunoglobulin". Efficiency of these assays both ELISA and hemagglutination test was found to be similar although hemagglutination is a semiquantitative procedure. The commercially available preparation exhibited some advantages; it is rapid, simple and reproducible. The hemagglutination test may be used as an express analysis in urgent medicine as well as in laboratories which do not possess special biochemical equipment.

Original Text (This is the original text for your reference.)

[Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test].

Optimal conditions were developed for estimation of myoglobin using immunoenzyme assay (ELISA) of competitive and "sandwich" types. Informative efficiency of the ELISA procedures was compared with commercially available hemagglutination test "Erythrocyte diagnosticum for myoglobin detection; dry immunoglobulin". Efficiency of these assays both ELISA and hemagglutination test was found to be similar although hemagglutination is a semiquantitative procedure. The commercially available preparation exhibited some advantages; it is rapid, simple and reproducible. The hemagglutination test may be used as an express analysis in urgent medicine as well as in laboratories which do not possess special biochemical equipment.

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G M, Rott G M, Lapshina I V, Rozhinskaia O A, Letova T V, Blinova,.[Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test].. 34 (5),124-9.

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