The recombinant strain of Salmonella typhimurium SL7207/pBMC-env, carrying a plasmid containing the gene of protein HIV-1 gp-160, was obtained under the monitoring by CMV-promoter. The above strain was used in the rectal and oral immunization of BALB/c mice. HIV-specific antibodies were detected in serum after a one-time immunization; such antibodies were able to inhibit the viral replication in vitro. Furthermore, the shaping-up of the specific cytotoxic and of proliferative responses was registered. Finally, the rectal immunization by cells of the Salmonella recombinant strain can be regarded as a promising delivery system of DNA-vaccine (pBMC-env), and it is more effective versus the oral immunization variant.

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hiv1 proliferative immunization dnavaccine pbmcenv

L I, Karpenko N A, Nekrasova G M, Ignat'ev A P, Agafonov T P, Proniaeva B V, Murashev A E, Romanovich N A, Klimov A P, Kozlov A A, Il'ichev,.[Immune response in oral and rectal immunization by the attenuated strain of salmonella carrying the HIV DNA-vaccine].. 48 (4),16-20.

L I, Karpenko N A, Nekrasova G M, Ignat'ev A P, Agafonov T P, Proniaeva B V, Murashev A E, Romanovich N A, Klimov A P, Kozlov A A, Il'ichev,"[Immune response in oral and rectal immunization by the attenuated strain of salmonella carrying the HIV DNA-vaccine]." 48

L I, Karpenko N A, Nekrasova G M, Ignat'ev A P, Agafonov T P, Proniaeva B V, Murashev A E, Romanovich N A, Klimov A P, Kozlov A A, Il'ichev,"[Immune response in oral and rectal immunization by the attenuated strain of salmonella carrying the HIV DNA-vaccine]."