Objective: To evaluate the preclinical value of 18 F-fluoropropionic acid ( 18 F-FPA) and 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography (PET) for imaging HCCs. Methods: The 18 F-FPA and 18 F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The 18 F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The 18 F-FPA PET imaging was performed in different tumor animal models and compared with 18 F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines. Results: In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of 18 F-FPA. The tumor-to-liver ratio of 18 F-FPA was superior to that of 18 F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of 18 F-FDG was higher than 18 F-FPA ( P < .01). FASN was highly expressed in cell lines with high 18 F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high 18 F-FDG uptake. The 18 F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions. Conclusions: PET imaging with 18 F-FPA combined with 18 F-FDG can be an alternative for detecting HCC.

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fasn imaging hccs detecting hcc radiotracer 18 ffdg uptake patterns ffluoropropionic acid matrix metalloproteinase2 glucose transporter1 glut1 fatty acid synthase fasn 18 ffpa pet imaging mmp2 ffluorodeoxyglucose ffdg positron emission tomography pet tumortoliver normalized

Jing Zhao MMed,Zhanwen Zhang MD,Dahong Nie MD,Hui Ma MD,Gongjun Yuan MD,Shu Su MMed,Shaoyu Liu DSc,Sheng Liu MD,Ganghua Tang DSc,.PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose. 18 (),.

Jing Zhao MMed,Zhanwen Zhang MD,Dahong Nie MD,Hui Ma MD,Gongjun Yuan MD,Shu Su MMed,Shaoyu Liu DSc,Sheng Liu MD,Ganghua Tang DSc,"PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose" 18

Jing Zhao MMed,Zhanwen Zhang MD,Dahong Nie MD,Hui Ma MD,Gongjun Yuan MD,Shu Su MMed,Shaoyu Liu DSc,Sheng Liu MD,Ganghua Tang DSc,"PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose"